A comparison study between new and conventional staining technique for peripheral blood and bone marrow smear with Wright’s stain.

Abstract

Background: The Wright’s stain is the most commonly used staining technique worldwide for the differential white blood cell counts and to study the morphology of red blood cells, platelets, and leukocytes in peripheral blood and bone marrow smear.
Objective: To comparison staining time, and efficiency between new and conventional staining technique for peripheral blood and bone marrow smear with Wright’s stain therefore physician can make a proper diagnosis and treatment in patients with hematology.
Methodology: The staining concentration was adjusted to an appropriate ratio and standardization.The score ofthe modification and conventional technique has been evaluated and scored by six pediatric hematologists independently.
Results: The samples of 50 blood smears and 25 bonemarrow smearsshowed a concentration of 0.36% staining was appropriate and practical in use. The modification stain shortened staining time with efficiency as in the conventional method. It takes only 4 minutes for blood smear result and 12 minutes for bone marrow smears, compared to conventional methods (8 minutes and 18 minutes), respectively.
Conclusion:Themodification stain shortened staining time with efficiency asin conventional method.