A Study of Hep Par 1 Antibody in Differential Diagnosis of Hepatocellular Carcinoma from Other Hepatic Tumors
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Abstract
Background: Many cases of hepatocellular carcinoma (HCC) have histologic pattern that are similar to cholangiocarcinoma (CCC) and metastatic adenocarcinoma. Histochemical and immunohistochemical studies are needed in these cases. A new antibody (Ab) Hep Par 1 is used in this study to evaluate its specificity to HCC and its relationship to degree of differentiation of the tumor. Material and Method: Paraffin blocks and slides of 37 cases of hepatic tumors from surgical pathology in Department of Pathology, Maharat Nakhon Ratchasima hospital had been retrieved. There were 16 HCC, 3 CCC, 14 metastatic adenocarcinoma, 2 metastatic squamous cell carcinoma, 1 malignant lymphoma, and 1 hepatoblastoma. All HCC cases were graded according to Edmondson and Steiner grading system. Immunohistochemical study for Hep Par 1 Ab using Streptavidin biotin method was done in all cases. Result: Hep Par 1 Ab was positive in 12/16 cases of HCC, 1/1 hepatoblastoma, and 1/14 metastatic adenocarcinoma. All the rest were negative. The positivity did not correlate with degree of differentiation of the tumor. Conclusion: Hep Par 1 Ab is specific and sensitive for HCC as seen in this study. It is a promising Ab in differential diagnosis of HCC from CCC and adenocarcinoma.
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