Cannabinoids analysis, pharmacokinetic prediction and antioxidant activity of elixir Thai traditional cannabis recipes
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Abstract
The Thai traditional medicinal recipes were used for treatment of several diseases from the past to the present, which have been recorded in the ancient Thai medicine recipe. Cannabis is one of the ingredients in the Thai medicinal recipes of King Narai the Great. In this study, the antioxidant was determined using by DPPH, ABTS and FRAP assays. The cannabinoid content was analyzed by HPLC. The 3 elixirs cannabis medical recipes which are Inthajaworn, Mahawattana, and Kaedhatuphikar, were determined TPC and TFC. In addition, the pharmacokinetic prediction of cannabinoids was studied. The results showed the Inthajaworn extract demonstrated the highest antioxidant activity with IC50 values of 54.21±1.81 µg/ml by ABTS assay. Mahawattana showed high activity with DPPH and FRAP assays. These 3 recipes showed high TPC and TFC values. The results from HPLC analysis showed CBDV and ∆9-THC as major compounds in each recipe. The pharmacokinetic prediction of cannabinoids using ADME method showed good pharmacokinetic properties and are permeable into the brain. The correlation analysis, the result showed that the antioxidant activity of
3 recipes showed a significant correlation with TPC and gallic acid content. In conclusion, antioxidant activity of 3 Thai traditional medicinal recipes made with cannabis correlated with total phenolic content (TPC) and total flavonoid content (TFC). Moreover, the result of this research can be developed from traditional medicinal recipes into pharmaceutical products.
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References
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