Determination of antimicrobial susceptibility profiles of Vibrio spp. isolated from Giant Freshwater Prawn samples in local market, Pathum-Thani Province
Keywords:
Vibrio spp., Giant freshwater prawn, Antimicrobial resistanceAbstract
This study aimed to assess the contamination of Vibrio spp. in giant freshwater prawn from a local aquatic food in Pathum Thani Province. The phenotypes, antimicrobial susceptibility and virulence factors of the bacterial isolates were also studied (In Vitro). Types of blood haemolysed and gelatin hydrolysis tests on culture medium were performed with all bacterial isolates. It was found that 10 Vibrio spp. isolates were isolated from 5 samples of giant freshwater prawn (5 prawns/sample). Biochemicals testing of bacterial isolates was conducted, the green colony on TCBS agar was classified as V. parahaemolyticus (6 isolates), whereas the yellow colony on TCBS agar was V. cholerae (4 isolates). The hemolysis of bacterial isolates were Alpha-hemolysis (60%) and Gamma-hemolysis (40%), whereas gelatin hydrolysis was negative (100%). Antimicrobial susceptibility test revealed that most isolates were resistant to 8 antibiotic discs tested (90-100%), except tetracycline, which 90% of the isolates exhibited an intermediate level of susceptibility. This study indicated that the antimicrobial susceptibility pattern of Vibrio spp. isolates tended to be resistant to gastrointestinal antibiotic treatment.
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