Antioxidant activity, total phenolic content and bioactivity of Momordica cochinchinensis (Lour.) Spreng. extract
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Abstract
This study aimed to evaluate the biological activities of aril extract from Momordica cochinchinensis, focusing on total phenolic content, inhibitory effects on tyrosinase and elastase enzymes, and cytotoxicity on human skin cells. The aril samples were carefully selected prior to extraction, which was then analyzed for total phenolic content. The results revealed that the extract exhibited antioxidant activity with a DPPH radical scavenging value of 1.51 ± 0.05 mg/mL compared to vitamin C as the standard. The total phenolic content was 13.18 ± 0.18 mg GAE/100 g DW. Cytotoxicity testing within the concentration range of 0.0001–1 mg/mL showed that the extract was non-toxic to human skin cells, with a cell viability of 95.35 ± 1.86% observed at the highest tested concentration (1 mg/mL). However, the extract exhibited no significant inhibitory effects on tyrosinase and elastase enzymes. These findings demonstrate the potential of M. cochinchinensis aril extract as a safe antioxidant agent for human skin, supporting its possible application as a bioactive ingredient in cosmetic or cosmeceutical products.
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Vuong T, Hirakawa M, Kato Y, Yamamoto S, Matoba Y. Gac oil: A comprehensive review of traditional uses, phytochemistry, and health benefits. J Funct Foods 2016;22:350-360.
Darapong P, Tachai S, Atthaisong J, Jaikla N, Rabiab B, Srimoon R. Tyrosinase inhibitory efficiency and antioxidant activity of
Gac fruit (Momordica cochinchinensis Spreng.) extract. RMUTP Res J Sci Technol 2023;17(2):149-156.
Le X, Pham M, Huynh-Bao M, Nguyen-Huynh H, Ha C. Polyphenol and elastase inhibitors extracted from medicinal plants in Vietnam. AIP Conf Proc 2023;2907(1):050005.
เสงี่ยม พงษ์บุญรอด. ไม้เทศเมืองไทย สรรพคุณของยาเทศและยาไทย. กรุงเทพฯ: โรงพิมพ์กรุงธน; 2522.
Lan H, Zhao B, Shen Y, Li X, Wang S, Zhang L, Zhang H. Phytochemistry, pharmacological activities, toxicity and clinical application of Momordica cochinchinensis. Curr Pharm Des 2019;25(6):715-728.
Sayeed J, Huynh T. Applications of natural biopesticides with a focus on trypsin inhibitors from Momordica cochinchinensis. Biopestic Int 2021;17(2):103.
Ishida B, Turner C, Chapman M, McKeon T. Anticancer potential of Momordica cochinchinensis Spreng. (gac fruit). J Agric Food Chem 2018;66(12):3450-3458.
Vadiveloo S, El-Enshasy H, Dailin D, Teong D, Jenol M, Marsin A, Manas N. Phytochemical compounds, therapeutic effects and antimicrobial properties of gac fruit (Momordica cochinchinensis Spreng.). J Bioproc Biomass Technol 2024;3(2):97-105.
Phrompittayarat W, Putalun W, Tanaka H, Jetiyanon K, Wittaya-areekul S, Ingkaninan K. Comparison of various extraction methods
of Bacopa monnieri. Naresuan Univ J. 2007;15(1):29-34.
Hou W, Lee M, Chen H, Liang W, Han C, Liu Y, Lin Y. Antioxidant activities of dioscorin, the storage protein of yam (Dioscorea batatas Decne) tuber. J Agric Food Chem 2001;49(10):4956-4960.
Blois M. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199-1200.
Manosroi A, Boonpisuttinant K, Winitchai S, Manosroi W, Manosroi J. Free radical scavenging and tyrosinase inhibition activity of physic nut (Jatropha curcas Linn.) seed oil entrapped in niosomes. Curr Nanosci 2011;7(5):825-829.
Miliauskas G, Venskutonis P, Beek T. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem 2004;85:231-237.
Vichai V, Kirtikara K. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat Protoc 2006;1(3):1112-1116.
Muhammad A, Pauzi N, Arulselvan P, Abas F, Fakurazi S. In vitro wound healing potential and identification of bioactive compounds from Moringa oleifera Lam. Biomed Res Int 2013;2013:974580.
Chang T. An updated review of tyrosinase inhibitors. Int J Mol Sci 2009;10(6):2440-2475.
Lee K, Kim J, Choi J, Choi J. Inhibitory effects of 150 plant extracts on elastase activity, and their anti-inflammatory effects. Int J Cosmet Sci 1999;21(2):71-82.
Abdul Rahman M, Ismail A, Azlan A, Abdul Aziz A, Muhammad N. Antioxidant and anti-adipogenic activities of Momordica cochinchinensis (Lour). Spreng fruit extracts. Malays J Med Health Sci 2024;20(3).
Popoviciu D, Pricop S, Radu M. Quantitative analysis of nutritional and bioactive compounds in bitter gourd (Momordica charantia L.) fruits. Ann Univ Craiova Biol Hortic Food Prod Process Technol Environ Eng 2023;28(64).
Nguyen D, Holien J, Dekiwadia C, Thrimawithana T, Piva T, Huynh T. Momordica cochinchinensis seed extracts induce apoptosis and necrosis in melanoma cells. Pharmaceuticals 2023;16(1):100.
Plyduang T, Sermkeaw N. Development and evaluation of a hydrogel containing Momordica cochinchinensis Spreng extract for topical applications. Braz J Pharm Sci 2022;58:e20130.
Kim J, Hong SC, Lee EH, Lee JW, Yang SH, Kim JC. Preventive effect of M. cochinchinensis on melanogenesis via tyrosinase activity inhibition and p-PKC signaling in melan-A cell. Nutrients 2021;13(11):3894.
Vaezi M. Structure and inhibition mechanism of some synthetic compounds and phenolic derivatives as tyrosinase inhibitors: Review and new insight. J Biomol Struct Dyn 2023;41(10):4798-4810.
Jakimiuk K, Gesek J, Atanasov AG, Tomczyk M. Flavonoids as inhibitors of human neutrophil elastase. J Enzyme Inhib Med Chem 2021;36(1):1016-1028.